Are there nonstop flights from Knoxville/Maryville (TYS) to Jacksonville (JAX)?

Yes. Allegiant Air fly this route. Flight time is about 1h 14m.

What is the fastest connection from Knoxville/Maryville (TYS) to Jacksonville (JAX)?

via Atlanta ≈ 1h 48m, via Charlotte ≈ 1h 57m, via Nashville ≈ 2h 12m (estimated flying time, excluding layovers).

from 12,000 JAL miles (economy, one way, based on the published award charts).

McGhee Tyson Airport (TYS) to Jacksonville International Airport (JAX)・629 km

Nonstop flights available (1 airline). Flight time about 1h 14m. Nonstop return flights also operate.

With milesJAL 12,000 miles+ (via CLT)Economy, one way, estimate. Tap a connection for miles by cabin.

7 one-stop options, sorted by estimated flying time. Tap one to see the airlines on each leg and booking links.

Times are estimated from distance. Check schedules, frequency and availability with the airline or a booking site.