Are there nonstop flights from Cusco (CUZ) to Puerto Maldonado (PEM)?

Yes. LATAM Airlines Peru, Sky Airline Peru fly this route. Flight time is about 52m.

Cusco → Puerto Maldonado

Alejandro Velasco Astete International Airport (CUZ) to Padre Aldamiz International Airport (PEM)・311 km

Nonstop flights available (2 airlines). Flight time about 52m. Nonstop return flights also operate.

Times are estimated from distance. Check schedules, frequency and availability with the airline or a booking site.